NKX2-2 Antibody

• Catalog Number: orb1252724
• Category: Antibodies
• Description: NKX2-2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NX2/294
• Tested applications: FC, IF, IHC
• Reactivity: Gallus, Human, Mouse, Rat
• Isotype: IgG2b, kappa
• Immunogen: Human NKX2.2 recombinant protein was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NKX2.2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NKX2-2
• UniProt ID: O95096
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox pr
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NKX2.2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of Ewing's sarcoma stained with NKX2.2 antibody (NX2/294).

IHC testing of human pancreas stained with NKX2.2 antibody (NX2/294).

IHC testing of FFPE rat pancreas with NKX2.2 antibody.