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NKB Rabbit Polyclonal Antibody

Catalog Number: orb11135

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11135
CategoryAntibodies
DescriptionNKB Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Human, Porcine
ReactivityMouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human NKB (81-90/121aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW1.3/13 kDa
TargetTAC3
UniProt IDQ9UHF0
RRIDAB_10746300
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesNeurokinin B; gamma tachykinin 3; Neurokinin B lik
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NoteFor research use only
Expiration Date12 months from date of receipt.
NKB Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (MOUSE spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NKB) Polyclonal Antibody, Unconjugated (orb11135) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NKB Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NKB) Polyclonal Antibody, Unconjugated (orb11135) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NKB Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NKB) Polyclonal Antibody, Unconjugated (orb11135) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NKB Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NKB Polyclonal Antibody, Unconjugated (orb11135) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

NKB Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NKB Polyclonal Antibody, Unconjugated (orb11135) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

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