NIX Antibody (Center)

• Catalog Number: orb29758
• Category: Antibodies
• Description: Peptide affinity purified rabbit polyclonal antibody.
• Target: BNIP3L
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: Synthetic Peptide
• UniProt ID: O60238
• MW: 23930
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, IF/ICC: 1:10-50, FACS: 1:10-50
• Antibody Type: Primary Antibody
• Clone Number: RB19646
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-BNIP3L, BNIP3A antibody, anti-BNIP3H antibody
• Research Area: Cancer Biology, Cardiovascular Research, Cell Biol
• Note: For research use only
• NCBI: NP_004322.1

Confocal immunofluorescent analysis of NIX Antibody (Center) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

NIX Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

NIX Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NIX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

NIX Antibody (Center) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the NIX antibody detected the NIX protein (arrow).