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Item 1 of 4
NIX Antibody (Center)
Catalog Number: orb1937954
| Catalog Number | orb1937954 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB19646 |
| Tested applications | FC, IF, IHC-P, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 23930 Da |
| Target | This NIX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the Central region of human NIX. |
| UniProt ID | O60238 |
| NCBI | NP_004322.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | BCL2/adenovirus E1B 19 kDa protein-interacting pro Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of NIX Antibody (Center) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
NIX Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NIX Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NIX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
NIX Antibody (Center) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the NIX antibody detected the NIX protein (arrow).
