NIPBL Antibody

• Catalog Number: orb1248589
• Category: Antibodies
• Description: NIPBL Antibody
• Target: NIPBL
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-RPDSPRVKQGDSNK
• UniProt ID: Q6KC79
• Tested applications: ELISA, IF, IHC
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NIPBL, Nipped-B homolog (Drosophila), CDLS, CDLS1,
• Research Area: Metabolism Research, Signal Transduction
• Note: For research use only
• NCBI: NP_597677.2, NP_056199.2

orb1248589 (2 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1248589 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

orb1248589 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing nuclear and cytoplasmic staining.