Nicastrin Antibody

• Catalog Number: orb1522952
• Category: Antibodies
• Description: Nicastrin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 659 and 709
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Nicastrin
• UniProt ID: Q92542
• NCBI: NP_056146.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: anterior pharynx-defective 2; ATAG1874; nicastrin
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Nicastrin by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody orb1522952 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Nicastrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody orb1522952 used for IP at 6 µg per reaction.