NGFB Rabbit Polyclonal Antibody

• Catalog Number: orb221814
• Category: Antibodies
• Description: NGFB Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinded human NGF beta full length protein
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 13/32 kDa
• Target: NGF
• UniProt ID: P01138
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NGF; beta nerve growth factor; NGF-beta; beta nerv
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Skin (Mouse) Lysate at 40 ug Lane 3: Thyroid gland (Mouse) Lysate at 40 ug Primary: Anti-NGFB (orb221814) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32/13 kD Observed band size: 35/13 kD.

Sample: Lane 1: Mouse Brain Lysates, Lane 2: Mouse Cerebellum Lysates, Lane 3: Human U-87 MG cell Lysates, Lane 4: Human SH-SY5Y cell Lysates, Lane 5: Human U-2 OS cell Lysates, Primary: Anti-NGFB (orb221814) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13/32kDa, Observed band size: 16/35kDa.

Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NGFB Polyclonal Antibody, Unconjugated (orb221814) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

U87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (orb221814) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.