NFATc1 antibody

• Catalog Number: orb11120
• Category: Tools
• Description: NFATc1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NFATc1 (601-716/716 aa)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, Flow-Cyt=1ug/test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 101 kDa
• Target: NFAT2
• UniProt ID: O95644
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: NFAT2; MGC138448; NF ATc; NF ATc1; NFAT 2; NFATc1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse lymphoma tissue (dilution at:1:200) using NFATc1 antibody

IHC-P of mouse hippocampal tissue using NFATc1 antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Anti

Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-NFAT2 antibody (orb11120) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol