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Catalog Number | orb1525376 |
---|---|
Category | Antibodies |
Description | NF45 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 340 and 390 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NF45 |
UniProt ID | Q12905 |
NCBI | NP_004506.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | interleukin enhancer binding factor 2, 45kDa; PRO3 Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse NF45 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NF45 antibody orb1525376 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of mouse NF45 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NF45 (Cat. No. orb1525376) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human NF45 by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-NF45 (Cat. No. orb1525376) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).
ELISA, IHC, WB | |
Canine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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