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    NF45 Antibody

    Catalog Number: orb1525376

    DispatchUsually dispatched within 5-10 working days
    $ 626.00
    Catalog Numberorb1525376
    CategoryAntibodies
    DescriptionNF45 Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIHC, IP, WB
    ReactivityHuman, Mouse
    Immunogenbetween 340 and 390
    Concentration1000 µg/ml
    Dilution rangeWB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
    Form/AppearanceWhole IgG
    ConjugationUnconjugated
    TargetNF45
    UniProt IDQ12905
    NCBINP_004506.2
    Storage2 - 8°C
    Buffer/PreservativesTris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
    Alternative namesinterleukin enhancer binding factor 2, 45kDa; PRO3
    Read more...
    NoteFor research use only
    Application notesFormat: Whole IgG
    Expiration Date12 months from date of receipt.
    NF45 Antibody

    Detection of human and mouse NF45 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NF45 antibody orb1525376 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

    NF45 Antibody

    Detection of mouse NF45 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NF45 (Cat. No. orb1525376) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

    NF45 Antibody

    Detection of human NF45 by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-NF45 (Cat. No. orb1525376) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).

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