ILF2 Antibody

• Catalog Number: orb1246705
• Category: Antibodies
• Description: ILF2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EKAYEKPPEKKEG
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 43kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1kDa according to NP_004506.2). Recommended concentration: 0.03-0.1ug/ml. This antibody has been successfully used in WB in the following papers: Ye J et al, RNA. 2017 Aug;23(8):1270-1284. PMID: 28487382. And Shamanna RA et al, Oncogene. 2013 Oct 24;32(43):5176-85 PMID: 23208500.Immunoprecipitation: This antibody was confirmed by a customer in a special IP procedure called PAR-CLIP (see for method PMID: 20644507). This data is from a previous batch, not on sale.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ILF2
• UniProt ID: Q12905
• NCBI: NP_004506.2, NP_001254738.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ILF2, NF45, interleukin enhancer binding factor 2,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 43kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1kDa according to NP_004506.2). Recommended concentration: 0.03-0.1ug/ml. This antibody has been successfully used in WB in the following papers: Ye J et al, RNA. 2017 Aug;23(8):1270-1284. PMID: 28487382. And Shamanna RA et al, Oncogene. 2013 Oct 24;32(43):5176-85 PMID: 23208500.Immunoprecipitation: This antibody was confirmed by a customer in a special IP procedure called PAR-CLIP (see for method PMID: 20644507). This data is from a previous batch, not on sale.
• Expiration Date: 12 months from date of receipt.

orb1246705 (0.03 ug/ml) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246705 (2.5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1246705 (2.5 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.