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Catalog Number | orb1527210 |
---|---|
Category | Antibodies |
Description | NF-YA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | between 320 and 333 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-NF-YA antibody A302-105A.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NF-YA |
UniProt ID | P23511 |
NCBI | NP_002496.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | HAP2 CCAAT-binding protein; HAP2; Transcription fa Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human NF-YA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF-YA antibody orb1527210 used for IP at 3 µg/mg lysate.
Detection of human NF-YA by immunohistochemistry.Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-NF-YA (Cat. No. orb1527210) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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