NF-YA Antibody

• Catalog Number: orb1527207
• Category: Antibodies
• Description: NF-YA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 333 and 347
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NF-YA
• UniProt ID: P23511
• NCBI: NP_002496.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: HAP2 CCAAT-binding protein; HAP2; Transcription fa
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse NF-YA by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-NF-YA antibody orb1527207 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human NF-YA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF-YA antibody orb1527207 used for IP at 3 µg/mg lysate.

Detection of human and mouse NF-YA by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-NF-YA (Cat. No. orb1527207) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB