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Catalog Number | orb344586 |
---|---|
Category | Antibodies |
Description | NF-Y antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Monkey |
Isotype | IgG |
Immunogen | Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
Concentration | 0.60 mg/mL |
Dilution range | ELISA: 1:10,000, WB: 1:1,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-NF-Y (a) antibody was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis. |
Conjugation | Unconjugated |
UniProt ID | P23511 |
NCBI | CAI20286.1 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-NF-Y Antibody, Nf-Y-A subunit, CAAT bo Read more... |
Note | For research use only |
Application notes | Anti-NF-Y antibody is tested for western blotting and suitable for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol using NF-Y antibody
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