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Item 1 of 3
NF-kB (phospho-65) ELISA Kit
Catalog Number: orb343760
| Catalog Number | orb343760 |
|---|---|
| Category | Assays and Kits |
| Description | NF-kB (phospho-65) ELISA Kit |
| Tested applications | ELISA |
| Reactivity | Human |
| Dilution range | ELISA: 1:100 |
| Conjugation | Unconjugated |
| Storage | See kit insert for complete instructions. |
| Alternative names | NF-kB Transcription Factor Kit, p65 kit, EIA kit, Read more... |
| Note | For research use only |
| Application notes | Rockland's NF-kB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p65) is detected by addition of a specific primary antibody directed against NF-kB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Rockland's NF-kB (p65) Transcription Factor Assay detects human NF-kB (p65). It will not cross-react with NF-kB (p50). |
| Expiration Date | Please enquire. |
Aβ-induced changes in the expressions of NF-κB p65, and effects of taxifolin and cilostazol in N2a Swe cells. E. Significant decrease in DNA binding activity of NF-κB by co-treatment with 10 µm taxifolin and 10 µm cilostazol as compared with taxifolin or cilostazol monotherapy. NF-κB p65 DNA binding activities were determined using a colorimetric NF-κB p65 transcription factor assay kit (p/n orb343760). Results are the means ± SEMs of 4 experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Vehicle (Veh); $ $ $P < 0.001 vs. Cilostazol alone; ††† P < 0.001 vs. 10 µm Taxifolin alone; ### P < 0.001 vs. Vehicle (Veh); @@@P < 0.001 vs. None.
NF-kB Sample Plate Format
Transcription factor assay absorbance of cell lysates isolated from stimulated (20 ng/mL TNFa for 30 min.) and non-stimulated HeLa cells demonstrating NF-?B (p50) activity.
