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Catalog Number | orb196257 |
---|---|
Category | Antibodies |
Description | NF-kB p65/RELA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human NF-kB p65 recombinant protein (Position: F99-S551). Human NF-kB p65 shares 86% amino acid (aa) sequence identity with mouse NF-kB p65. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 60219 MW |
UniProt ID | Q04206 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Transcription factor p65;Nuclear factor NF-kappa-B Read more... |
Note | For research use only |
Application notes | WB: The detection limit for NF-kB p65 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody.Lane 1:human HeLa cell;2:human HepG2 cell;3:human Caco-2 cell;4:human Raji cell.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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