NF-kB p65/RELA Antibody

• Catalog Number: orb443125
• Category: Antibodies
• Description: NF-kB p65/RELA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human NF-kB p65 recombinant protein (Position: F99-S551).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 65 kDa
• UniProt ID: Q04206
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transcription factor p65; Nuclear factor NF-kappa-
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of NF-kB p65 using anti-NF-kB p65 antibody.Lane 1:human COLO-320 cell;2:human A549 cell;3:human HepG2 cell;4:human MDA-MB-231 cell;5:human PANC-1 cell;6:human A375 cell.

WB analysis of NF-kB p65 using anti-NF-kB p65 antibody.Lane 1:rat spleen tissue;2:rat lung tissue;3:rat kidney tissue;4:rat testis tissue;5:mouse spleen tissue;6:mouse lung tissue;7:mouse testis tissue;8:mouse NIH3T3 cell.

IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody.NF-kB p65 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody.NF-kB p65 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody.NF-kB p65 was detected in paraffin-embedded section of rat kidney tissue.

IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody.NF-kB p65 was detected in paraffin-embedded section of rat small intestine tissue.