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Neutrophil Elastase Rabbit Polyclonal Antibody

Catalog Number: orb500993

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb500993
CategoryAntibodies
DescriptionNeutrophil Elastase Rabbit Polyclonal Antibody
TargetELANE
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human Neutrophil Elastase/ELANE (101-200/267aa)
UniProt IDP08246
MW26 kDa
Tested applicationsFC
Dilution rangeFlow-Cyt=1μg/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesBone Marrow Serine Protease; ELA 2; ELA2; ELANE; E
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NoteFor research use only
Neutrophil Elastase Rabbit Polyclonal Antibody

Blank control: A549 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Neutrophil Elastase Rabbit Polyclonal Antibody

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-Neutrophil Elastase antibody (orb500993), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

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