Neuropeptide Y Rabbit Polyclonal Antibody

SKU: orb11154

Description

Neuropeptide Y Rabbit Polyclonal Antibody

Research Area

Angiogenic Factors, Cardiovascular Research, Heart Function, Hormone Research, Inotropics, Metabolism, Neuropeptides, Neuroscience, Varscular, Vasoconstriction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Human, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NPY (29-64/97aa)
• Target: NPY
• Molecular Weight: 4/8 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Neuropeptide tyrosine; Neuropeptide Y precursor; NPY; PYY 4; PYY4; Y Neuropeptide; Pro-neuropeptide Y; NPY_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Neuropeptide Y) Polyclonal Antibody, Unconjugated (orb11154) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated (orb11154) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

NPY

References

D Massoud, AF El-kott, MA AlShehri, ER Elbealy Microscopic investigation of the exocrine and endocrine pancreas in the southern white-breasted hedgehog (Erinaceus concolor) Zoomorphology,, (2024)

PubMed:

Applications

IHC

Nader Goodarzi 1, Ayda Bashiri Histology and immunofluorescent study of the pancreas in lovebird (Agapornis personatus) Vet Med Sci, 10, e1394 (2024)

PubMed: 38459816

Applications

IF