Neuropeptide Y Rabbit Polyclonal Antibody

• Catalog Number: orb11154
• Category: Antibodies
• Description: Neuropeptide Y Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Equine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NPY (29-64/97aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 4/8 kDa
• Target: NPY
• RRID: AB_10752667
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Neuropeptide tyrosine; Neuropeptide Y precursor; N
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

D Massoud, AF El-kott, MA AlShehri, ER Elbealy Microscopic investigation of the exocrine and endocrine pancreas in the southern white-breasted hedgehog (Erinaceus concolor) Zoomorphology,, (2024)

PubMed:

Applications

IHC

Nader Goodarzi 1, Ayda Bashiri Histology and immunofluorescent study of the pancreas in lovebird (Agapornis personatus) Vet Med Sci, 10, e1394 (2024)

PubMed: 38459816

Applications

IF

Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Neuropeptide Y) Polyclonal Antibody, Unconjugated (orb11154) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated (orb11154) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.