Neurokinin A Rabbit Polyclonal Antibody

• Catalog Number: orb500956
• Category: Antibodies
• Description: Neurokinin A Rabbit Polyclonal Antibody
• Target: TAC1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human neurokinin A/neuropeptide K (98-107/129aa)
• UniProt ID: P20366
• MW: 11/13 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Neurokinin alpha; Neurokinin K; NeurokininA; Neuro
• Research Area: Cancer Research, Hormone Research, Metabolism, Neu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Spleen tissue lysates, Lane 2: Mouse Stomach tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Human U-2 OS cell lysates, Lane 5: Human SH-SY5Y cell lysates, Lane 6: Human u251 cell lysates, Primary: Anti-Neurokinin A (orb500956) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11/13 kDa, Observed band size: 11 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NKA/Neurokinin A Polyclonal Antibody, Unconjugated (orb500956) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NKA/Neurokinin A Polyclonal Antibody, Unconjugated (orb500956) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.