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Neurokin B receptor Rabbit Polyclonal Antibody

Catalog Number: orb11138

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11138
CategoryAntibodies
DescriptionNeurokin B receptor Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Canine, Equine, Rabbit, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human NKR (151-250/440aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, ELISA=1:5000-10000
Form/AppearanceLiquid
ConjugationUnconjugated
MW52 kDa
TargetTACR3
UniProt IDP29371
RRIDAB_10751832
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesMGC148060; MGC148061; Neurokinin B receptor; Neuro
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NoteFor research use only
Expiration Date12 months from date of receipt.
Neurokin B receptor Rabbit Polyclonal Antibody

Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-Neurokin B receptor antibody (orb11138), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, Primary antibody (orb11138, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Neurokin B receptor Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Neurokin B receptor) Polyclonal Antibody, Unconjugated (orb11138) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Neurokin B receptor Rabbit Polyclonal Antibody

Tissue/Cell: Mouse lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NK3R Polyclonal Antibody, Unconjugated (orb11138) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.