Neurogenin3 Antibody (N-term)

SKU: orb1931803

Description

Neurogenin3 Antibody (N-term)

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IHC-P - 1:100-500, WB - 1:1000, IF - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 23077 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATOH5, BHLHA7, NGN3

Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Staining Neurogenin3 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining Neurogenin3 in human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution + THP-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Neurogenin3 Antibody (N-term) at 1:500 dilution + Human placenta tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution. Lane 1: DU145 whole cell lysate. Lane 2: LNCap whole cell lysate. Lane 3: PANC-1 whole cell lysate. Lane 4: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q9Y4Z2