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Neurogenin3 Antibody (N-term)

Catalog Number: orb1931803

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1931803
CategoryAntibodies
DescriptionPurified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB02913
Tested applicationsIF, IHC-P, WB
ReactivityHuman, Mouse
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeIF: 1:10~50, WB: 1:2000, WB: 1:500, WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW23077 Da
TargetThis Neurogenin3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human Neurogenin3.
UniProt IDQ9Y4Z2
NCBINP_066279.2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesNeurogenin-3, NGN-3, Class A basic helix-loop-heli
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NoteFor research use only
Expiration Date12 months from date of receipt.
Neurogenin3 Antibody (N-term)

Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Neurogenin3 Antibody (N-term)

Staining Neurogenin3 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Neurogenin3 Antibody (N-term)

Staining Neurogenin3 in human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Neurogenin3 Antibody (N-term)

Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution + THP-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Neurogenin3 Antibody (N-term)

Anti-Neurogenin3 Antibody (N-term) at 1:500 dilution + Human placenta tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Neurogenin3 Antibody (N-term)

All lanes: Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution. Lane 1: DU145 whole cell lysate. Lane 2: LNCap whole cell lysate. Lane 3: PANC-1 whole cell lysate. Lane 4: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Neurogenin3 Antibody (N-term) [orb1167439]

    IF,  IHC-P,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 30 μl