Neurofilament medium protein antibody

• Catalog Number: orb44467
• Category: Antibodies
• Description: Mouse Monoclonal to Neurofilament medium protein.
• Clonality: Monoclonal
• Clone Number: NF-09
• Tested applications: ICC, IHC-P, WB
• Reactivity: Mammal
• Isotype: Mouse IgG2a
• Immunogen: Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
• Concentration: 1 mg/ml
• Dilution range: Immunocytochemistry: Positive tissue: Neuro2A murine cell line, Carnoys fixative 2 x 3 min, blocking 1% glycine + 0.2% gelatin 10 min. Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Neurofilament M
• Entrez: 4741
• UniProt ID: P07197
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Neurofilament medium protein
• Note: For research use only
• Application notes: Immunocytochemistry: Positive tissue: Neuro2A murine cell line, Carnoys fixative 2 x 3 min, blocking 1% glycine + 0.2% gelatin 10 min. Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry staining of neurofilament medium protein in murine Neuro2A cells by antibody NF-09 conjugated with Dyomics 547 (red). DNA stained by Hoechst (blue).

Western blotting analysis of human neurofilament M protein using mouse monoclonal antibody NF-09 on lysates of HEK-293 cell line, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-neurofilament M monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament M was detected around 160 kDa.