Neurofilament L Rabbit Polyclonal Antibody

SKU: orb1294399

Description

Rabbit polyclonal antibody to Neurofilament L

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: Western Blot 1:1000-1: 2000, Immunofluorescence 1:200-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Recombinant fragment corresponding to Human Neurofilament L. Encompassing a sequence within the C-terminus region.
• Target: Neurofilament L / NF L
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: anti-Neurofilament-L / NF-L antibody at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294399 overnight at 4°C.

Immunofluorescent analysis. Sample: primary cortical neurons, NF-L antibody (orb1294399): 1-250, Red: Tuj1 antibody, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.

Quick Database Links

Gene Symbol

Neurofilament L / NF L