Neurofilament H/NF H Antibody

SKU: orb1294401

Description

Rabbit polyclonal antibody to Neurofilament H

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: Western Blot 1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry ( Frozen) 1:200-1:400 Immunohistochemistry ( Paraffin) 1:200-1:400
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region.
• Target: Neurofilament H / NF H

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

All lanes: Anti-NF-H antibody at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294401 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294401 anti-NF-H antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescent analysis. Sample: primary cortical neurons, NF-H antibody (orb1294401): 1-250, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.

Immunohistochemical of frozen sections. NF-H antibody (orb1294401): 1-250, Anti-rabbit 488: 1-500.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. NF-H antibody (orb1294401): 1-250, Anti-rabbit 488: 1-500.

Quick Database Links

Gene Symbol

Neurofilament H / NF H