Neurofilament H / NF H

• Catalog Number: orb1294401
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Neurofilament H
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region.
• Dilution range: Western Blot 1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry ( Frozen) 1:200-1:400 Immunohistochemistry ( Paraffin) 1:200-1:400
• Conjugation: Unconjugated
• Target: Neurofilament H / NF H
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-NF-H antibody at 1/1000 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294401 overnight at 4°C.

Immunofluorescent analysis. Sample: primary cortical neurons; NF-H antibody (orb1294401): 1-250; Anti-rabbit 488: 1-500; Fixed: 4% paraformaldehyde at RT for 20 min.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. NF-H antibody (orb1294401): 1-250; Anti-rabbit 488: 1-500.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294401 Anti-NF-H antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical of frozen sections. NF-H antibody (orb1294401): 1-250; Anti-rabbit 488: 1-500.