NEUROD1 Rabbit Polyclonal Antibody

• Catalog Number: orb11114
• Category: Antibodies
• Description: NEUROD1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Human, Porcine
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NEUROD1 (21-120/356aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 40 kDa
• Target: NEUROD1
• UniProt ID: Q13562
• RRID: AB_10750515
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: atonal; Neurod1 protein; basic helix loop helix tr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-Neuro D1/AF647 Conjugated antibody, Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF647. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Cerebellum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-NeuroD1 (orb11114) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 49 kD.

Sample: Lane 1: Stomach (Mouse) Lysate at 40 ug, Lane 2: Spinal cord (Mouse) Lysate at 40 ug, Primary: Anti-NeuroD1 (orb11114) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 49 kD.