Neuritin Rabbit Polyclonal Antibody

SKU: orb13605

Description

Neuritin Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Neuritin (51-142/142aa)
• Target: NRN1
• Molecular Weight: 16 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Neuritin 1; NRN; NRN1; OTTHUMP00000015989; RP3 380B8.2 protein; NRN1_HUMAN.

Blank control: U251. Primary Antibody (green line): Rabbit Anti-Neuritin antibody (orb13605), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Brain (Mouse) lysate at 30 ug, Primary: Anti-Neuritin (orb13605) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000 dilution, Predicted band size: 16 kD, Observed band size: 20 kD.

Quick Database Links

Gene Symbol

NRN1

UniProt

Q9NPD7