NET1 antibody

• Catalog Number: orb19257
• Category: Antibodies
• Description: Goat polyclonal antibody to NET1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 2.5µg/ml
• Conjugation: Unconjugated
• MW: 67.7; 61.9
• Target: NET1 / ARHGEF8 (Internal)
• Entrez: 10276
• Protein Sequence: DKLEYLDEKQRDPR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NET1 antibody, anti ARHGEF8 antibody, anti ne
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19257 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.