NET1 antibody

• Catalog Number: orb18601
• Category: Antibodies
• Description: Goat polyclonal antibody to NET1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:128000, IHC-P: 1-2µg/ml
• Conjugation: Unconjugated
• MW: 67.7; 61.9
• Target: NET1 / ARHGEF8 (C Terminus)
• Entrez: 10276
• Protein Sequence: DKALSGGKRKETLV
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NET1 antibody, anti ARHGEF8 antibody, anti NE
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Tonsil shows staining of fibrous structures. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave bands at approx 85kDa in Human Placenta and a very strong band at approx 40-50kDa in Human Skeletal Muscle lysates after 0.1 μg/ml staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 67.7kDa according to NP_001040625.1 and of 61.9kDa according to NP_005854.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb18601 (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.