Nestin Antibody (C-term)

• Catalog Number: orb1931834
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1560~1590 amino acids from the C-terminal region of human nestin.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P48681
• MW: 177439 Da
• Tested applications: IF, IHC-P
• Dilution range: IF: 1:10~50, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB2857
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nestin, NES
• Note: For research use only
• NCBI: NP_006608.1

Immunofluorescence analysis of anti-Nestin Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.