Neptune Sample Diluent

• Catalog Number: orb623172
• Category: Assays and Kits
• Description: A non-mammalian protein-containing solution highly recommended for use with serum or plasma samples from mouse, porcine or bovine in an antigen-down format. Also suitable for human or other mammalian plasma samples in antibody-sandwich ELISAs.
• Concentration: 1X
• Application notes: 1. AO may be used neat or diluted in diH2O, PBS, or media. ICT’s AO is supplied at 0.5 mL liquid at 1 mM (266 µg/mL). 2. Add AO to the cell sample media at 0.5-5 µM, equal to a final dilution of 1:2,000 – 1:200 in the cells (.05-.5% v/v). For example, if using AO at 1.0 µM in the final cell suspension, it must be diluted 1:1,000. First dilute it 1:100 in PBS or diH2O; e.g., put 10 µL AO into 990 µL PBS or diH2O. Pipette the diluted AO into the cell suspension at approximately 1:10; e.g., put 50 µL diluted AO into 450 µL cell suspension., 3. Incubate 15-30 minutes at 37°C., 4. Wash cells if reagent is too bright., 5. Analyze with fluorescence. Lysosomes will appear yellowish green by illuminating cells with a blue light (488 nm) excitation filter and a green light (540-550 nm) emission/barrier filter. Alternatively, lysosomes will appear red when using an excitation filter of 550 nm (540-560 nm) and a long pass >610 nm emission/barrier filter.
• Storage: 2-8°C
• Note: For research use only
• Expiration Date: Please enquire.