NEMO antibody

• Catalog Number: orb420274
• Category: Antibodies
• Description: NEMO antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-NEMO was affinity purified from whole rabbit serum prepared by repeated immunizations with a recombinant protein of human NEMO.
• Concentration: 1.1 mg/ml
• Dilution range: ELISA: 1:50,000 - 1:400,000, IP: 5µg, WB: 1:500-1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-NEMO is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody detects human NEMO. Cross reactivity with NEMO from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: Q9Y6K9
• NCBI: NP_003630.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-NEMO antibody, IKBKG Antibody, NF-kapp
• Note: For research use only
• Application notes: Anti-NEMO antibody has been tested by western blot and is suitable for immunoprecipitation and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48kDa in size corresponding to endogenous NEMO protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.