Optineurin/OPTN Antibody

• Catalog Number: orb251526
• Category: Antibodies
• Description: Optineurin/OPTN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Optineurin recombinant protein (Position: R241-I577). Human Optineurin shares 82% amino acid (aa) sequence identity with both mouse and rat Optineurin.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 65921 MW
• UniProt ID: Q96CV9
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Optineurin;E3-14.7K-interacting protein;FIP-2;Hunt
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-Optineurin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of Optineurin using anti-Optineurin antibody.Lane 1:human HeLa cell; 2:human HT1080 cell; 3:huamn HepG2 cell; 4:rat heart tissue;5:rat brain tissue; 6:mouse heart tissuel lysates; 7:mouse brain tissue.

IHC analysis of Optineurin using anti-Optineurin antibody. Optineurin was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue.

IHC analysis of Optineurin using anti-Optineurin antibody. Optineurin was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of Optineurin using anti-Optineurin antibody. Optineurin was detected in a paraffin-embedded section of rat brain tissue.