NEK1 Antibody

• Catalog Number: orb1262773
• Category: Antibodies
• Description: NEK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This NEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1165-1196 amino acids from the C-terminal region of human NEK1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 143 kDa
• Target: NEK1
• UniProt ID: Q96PY6
• NCBI: Q96PY6
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Serine/threonine-protein kinase Nek1, Never in mit
• Note: For research use only
• Application notes: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:500 dilution + human fetal kidney lysate Lysates/proteins at 20 ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.