NEFH Antibody

• Catalog Number: orb1252726
• Category: Antibodies
• Description: NEFH Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NF421
• Tested applications: FC, IHC, WB
• Reactivity: Gallus, Human, Mouse, Porcine, Rat
• Isotype: IgG1
• Immunogen: Human recombinant Neurofilament protein was used as the immunogen. (1)
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (2)Prediluted format : incubate for 30 min at RT (3)The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.1. The NF421 antibody is specific to the heavy subunit.2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NEFH
• UniProt ID: P12036
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: NEFH, 200 kDa neurofilament protein, KIAA0845, NFH
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (2)Prediluted format : incubate for 30 min at RT (3)The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.1. The NF421 antibody is specific to the heavy subunit.2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Human cerebellum stained with Neurofilament antibody (NF421).

SDS-PAGE Analysis of Purified, BSA-Free Neurofilament Antibody (clone NF421). Confirmation of Integrity and Purity of the Antibody.