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UBE2F Rabbit Polyclonal Antibody

SKU: orb3094266

Description

Anti-UBE2F Antibody -1. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as , ensuring unmatched performance.

Images & Validation

Tested ApplicationsELISA, FC, ICC, IHC, WB
Dilution RangeWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
ReactivityHuman, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived human UBE2F recombinant protein (Position: M1-R185). Human UBE2F shares 96.8% and 96.2% amino acid (aa) sequence identity with mouse and rat UBE2F, respectively.
TargetNEDD8-conjugating enzyme UBE2F
Molecular Weight21 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageAt -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

NCE2; EC:2.3.2.32; NEDD8-conjugating enzyme 2; NEDD8 protein ligase UBE2F; NEDD8 conjugating enzyme 2

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

UBE2F Rabbit Polyclonal Antibody (orb3094266)

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Available Sizes

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100 μg
$ 450.00
DispatchUsually dispatched within 2-4 weeks
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