NEDD8 Antibody (N-term)

• Catalog Number: orb1937612
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This NEDD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-32 amino acids from the N-terminal region of human NEDD8.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rabbit, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q15843
• MW: 9072 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB0639
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: NEDD8, Neddylin, Neural precursor cell expressed d
• Note: For research use only
• NCBI: NP_006147.1

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of NEDD8 polyclonal antibody in HeLa cell lysate. NEDD8 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.