NECAB1 Rabbit Polyclonal Antibody

• Catalog Number: orb157941
• Category: Antibodies
• Description: NECAB1 Rabbit Polyclonal Antibody
• Target: NECAB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Canine, Equine, Human, Mouse, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human NECAB1/EF-CBP1 (51-150/351aa)
• UniProt ID: Q8N987
• MW: 41 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EF-CBP1; EF-hand calcium-binding protein 1; EFCBP1
• Note: For research use only

Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NECAB1 Polyclonal Antibody, Unconjugated (orb157941) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.