NDUFB5 Rabbit Polyclonal Antibody

SKU: orb221674

Description

NDUFB5 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Gallus, Human, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NDUFB5 (61-150/189aa)
• Target: NDUFB5
• Molecular Weight: 17 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CI SGDH; CI-SGDH; Complex I SGDH; Complex I-SGDH; DKFZp686N02262; FLJ30597; MGC111204; MGC12314; mitochondrial; NADH dehydrogenase(ubiquinone) 1 beta subcomplex 5; NADH dehydrogenase(ubiquinone) 1 beta subcomplex 5 16kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5 mitochondrial; NADH ubiquinone oxidoreductase SGDH subunit; NADH-ubiquinone oxidoreductase SGDH subunit; NDUB5_HUMAN; Ndufb5; SGDH.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NDUFB5) Polyclonal Antibody, Unconjugated (orb221674) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NDUFB5) Polyclonal Antibody, Unconjugated (orb221674) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

NDUFB5

UniProt

O43674