NDUFA8 Rabbit Polyclonal Antibody

SKU: orb6129

Description

NDUFA8 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Complex I, Energy Metabolism, Metabolism, Mitochondrial, Mitochondrial Markers, Oxidative Stress, Respiratory Disease

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NDUFA8 (101-172/172aa)
• Target: NDUFA8
• Molecular Weight: 19 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Complex I 19kD; CI 19KD; CI PGIV; Complex I 19k; Complex I PGIV; MGC793; NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 8(19kD PGIV); NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 8 19kDa; NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 8; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; NADH ubiquinone oxidoreductase 19 kDa subunit; NADH ubiquinone oxidoreductase PGIV subunit; NDUFA 8; PGIV.

Tissue/Cell: mouse brain tissue 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NDUFA8 Polyclonal Antibody, Unconjugated (orb6129) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

NDUFA8

UniProt

P51970