NCR3 Rabbit Polyclonal Antibody

• Catalog Number: orb13335
• Category: Antibodies
• Description: NCR3 Rabbit Polyclonal Antibody
• Target: NCR3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Equine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CD337 (81-180/201aa)
• UniProt ID: O14931
• RRID: AB_10749558
• MW: 20 kDa
• Tested applications: FC
• Dilution range: Flow-Cyt=2ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Natural cytotoxicity triggering receptor 3; 1C7; A
• Research Area: Innate Immunity, Natural Killer Cell Lineage
• Note: For research use only

Black line: Positive blank control (HL60), Negative blank control (HUVEC), Green line: Primary Antibody (Rabbit Anti-NCR3 antibody (orb13335)), Orange line: Isotype Control Antibody (Rabbit IgG). Blue line: Secondary Antibody (Goat anti-rabbit IgG-AF647), HL60 (Positive) and HUVEC (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NCR3 Antibody (orb13335) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Blank control: HL60. Primary Antibody (green line): Rabbit Anti-NCR3 antibody (orb13335), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.