NCF4 antibody

• Catalog Number: orb19549
• Category: Antibodies
• Description: Goat polyclonal antibody to NCF4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 39.0, 38.8
• Target: NCF4 / P40PHOX
• Entrez: 4689
• Protein Sequence: KDFPEEDDPTN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NCF4 antibody, anti P40PHOX antibody, anti ne
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4ug/ml.WB: Approx. 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.0kDa according to NP_000622.2 and 38.8kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19549 (0.5ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing P40PHOX (RC201191) and probed with orb19549 (mock transfection in first lane), tested by Origene.

orb19549 (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.