NCF1 antibody

• Catalog Number: orb215428
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NCF1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S370 of human p47 phox protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NCF1
• Entrez: 653361, 17969
• UniProt ID: Q09014, P14598
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti NOXO2 antibody, anti SH3PXD1A antibody, anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of p47 phox (Phospho-S370) expression in HEK293T (A), mouse lung (B), mouse liver (C), rat lung (D), rat liver (E) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 47 kD)

Immunohistochemical analysis of p47 phox (Phospho-S370) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-p47 phox (Phospho-S370) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.