NCF1 Antibody

SKU: orb1247936

Description

NCF1 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse, Porcine
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:20000.Western Blot:Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates (calculated MW of 44.7kDa according to Human NP_000256.4, 46.0kDa according to Mouse NP_001272966.1 and 45.3kDa according to Pig NP_001106691.1). The observed molecular weights correspond to different antibodies from other commercial sources. Recommended concentration: 0.01-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate. Preliminary testing was unsuccessful on Rat Spleen and Thymus for this particular batch.Immunohistochemistry:Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SESTKRKLASAV
• Target: NCF1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2

orb1247936 (1 ug/ml) staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247936 (0.2 ug/ml) staining of U937 (A), (0.01 ug/ml) Daudi (B), (0.3 ug/ml) U251 (C) and negative control A431 (D) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247936 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247936 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1247936 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

NCF1

UniProt

P14598

RefSeq (Protein):NP_000256.4