NCF1 Antibody

• Catalog Number: orb1247936
• Category: Antibodies
• Description: NCF1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse, Porcine
• Immunogen: The immunogen for this antibody is: C-SESTKRKLASAV
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:20000.Western Blot:Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates (calculated MW of 44.7kDa according to Human NP_000256.4, 46.0kDa according to Mouse NP_001272966.1 and 45.3kDa according to Pig NP_001106691.1). The observed molecular weights correspond to different antibodies from other commercial sources. Recommended concentration: 0.01-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate. Preliminary testing was unsuccessful on Rat Spleen and Thymus for this particular batch.Immunohistochemistry:Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NCF1
• UniProt ID: P14598
• NCBI: NP_000256.4
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, n
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:20000.Western Blot:Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates (calculated MW of 44.7kDa according to Human NP_000256.4, 46.0kDa according to Mouse NP_001272966.1 and 45.3kDa according to Pig NP_001106691.1). The observed molecular weights correspond to different antibodies from other commercial sources. Recommended concentration: 0.01-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate. Preliminary testing was unsuccessful on Rat Spleen and Thymus for this particular batch.Immunohistochemistry:Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247936 (1 ug/ml) staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247936 (0.2 ug/ml) staining of U937 (A), (0.01 ug/ml) Daudi (B), (0.3 ug/ml) U251 (C) and negative control A431 (D) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247936 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247936 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1247936 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).