NCC Antibody

• Catalog Number: orb67566
• Category: Antibodies
• Description: Rabbit Anti-Rat NCC Polyclonal
• Target: NCC
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Canine, Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Affinity Purified
• Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
• UniProt ID: P55018, P55017
• MW: 160kDa
• Tested applications: ICC, IEM, IF, IHC, WB
• Dilution range: WB (1:1000), IHC-P (1:200)
• Application notes: 1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SLC12A3 Antibody, SCYL1 Antibody,CKb10 Antibody, M
• Research Area: Cancer Biology, Neuroscience, Pharmacology & Drug
• Note: For research use only
• Entrez: 54300, 6559
• NCBI: NP_000330, NP_062218
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000.

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Mouse. Fixation: paraformaldehyde-fixed paraffin-embedded. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:500 for Overnight at 4°C. Secondary Antibody: Biotinylated Goat Anti-Rabbit IgG at 1:500 for 30 min at RT.

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Mouse. Fixation: paraformaldehyde-fixed paraffin-embedded. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000 for Overnight at 4°C. Secondary Antibody: Biotinylated Goat Anti-Rabbit IgG at 1:501 for 30 min at RT.