NCC Antibody

SKU: orb67566

Description

Rabbit Anti-Rat NCC Polyclonal

Research Area

Cancer Biology, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IEM, IF, IHC, WB
• Dilution range: WB (1:1000), IHC-P (1:200)
• Reactivity: Canine, Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
• Target: NCC
• Molecular Weight: 160kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

NCC, SLC12A3, SCYL1, CKb10, MCP-4, MGC17134, NCC-1, NCC1, SCYA13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, New CC chemokine 1, Small inducible cytokine A13, Small inducible cytokine subfamily A (Cys-Cys) member 13, Chemokine (C-C)

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000.

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Mouse. Fixation: paraformaldehyde-fixed paraffin-embedded. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:500 for Overnight at 4°C. Secondary Antibody: Biotinylated Goat Anti-Rabbit IgG at 1:500 for 30 min at RT.

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Mouse. Fixation: paraformaldehyde-fixed paraffin-embedded. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000 for Overnight at 4°C. Secondary Antibody: Biotinylated Goat Anti-Rabbit IgG at 1:501 for 30 min at RT.

Quick Database Links

Gene Symbol

NCC

UniProt

P55018, P55017

NCBI Gene ID

54300, 6559

RefSeq (Protein):NP_000330, NP_062218