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Catalog Number | orb1959087 |
---|---|
Category | Proteins |
Description | NCAM1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 66.1 kDa and the accession number is P13591-3. |
Tag | C-His |
Purity | 96.90% |
UniProt ID | P13591 |
MW | 66.1 kDa (predicted) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | NCAM1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 66.1 kDa and the accession number is P13591-3. |
Expression Region | A DNA sequence encoding the human NCAM1 (NP_001070150.1) (Met1-Pro603) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 20 |
Storage | -20°C |
Note | For research use only |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 77.0 kDa after removal of the signal peptide.The apparent molecular mass of CD56-His is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
> 90% as determined by SDS-PAGE | |
65.7 kDa |
98.00% | |
67.9 kDa (predicted) |
98.00% | |
78.48 kDa (predicted). Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result. |
Greater than 95.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |