Nav1.8 Antibody (APC)

SKU: orb148840

Description

Mouse monoclonal to Nav1 (APC). 8. Nav1. 8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1. 8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1. 8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals..

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: AM, ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-342 was sufficient for detection of Nav1.8 in 10 µg of COS cell lysate transiently expressing Nav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG2a
• Clone No.: S134
• Immunogen: Fusion protein amino acids 1724-1956 of rat Nav1.8
• Target: Nav1.8
• Molecular Weight: 220kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nav1.8, SCN10A, Scn10a, PN3, mPN3, Sns, SNS, Peripheral nerve sodium channel 3, Pn3 (gene name), Sensory neuron sodium channel, Sodium channel protein type 10 subunit alpha, Sodium channel protein type X alpha subunit, Voltage-gated sodium channel alpha subunit Nav1.8

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav1.8 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic staining and some dull nuclear staining.

Immunohistochemistry analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Heavy filaggrin-like staining, lower epidermal cells have some staining.

Quick Database Links

Gene Symbol

Nav1.8

UniProt

Q62968

NCBI Gene ID

29571

RefSeq (Protein):NP_058943.1