NARS Antibody

• Catalog Number: orb1270389
• Category: Antibodies
• Description: NARS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Monkey, Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This NARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human NARS.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 63 kDa
• Target: NARS
• UniProt ID: O43776
• NCBI: O43776
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Asparagine--tRNA ligase, cytoplasmic, Asparaginyl-
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NARS using rabbit polyclonal NARS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NARS gene.

NARS Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in WiDr, HepG2 cell line lysates (35 ug/lane).

Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.