NARG1 Antibody

• Catalog Number: orb1527133
• Category: Antibodies
• Description: NARG1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 816 and 866
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:50 - 1:250
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NARG1
• UniProt ID: Q9BXJ9
• NCBI: NP_476516.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: TBDN100; Ga19; transcriptional coactivator tubedow
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse NARG1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50µg) cells. Antibodies: Affinity purified rabbit anti-NARG1 antibody orb1527133 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NARG1 was also immunoprecipitated, albeit poorly, by rabbit anti-NARG1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Detection of human NARG1 by immunohistochemistry.Sample: FFPE sections of human testicular seminoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti-NARG1 (Cat. No. orb1527133) used at a dilution of 1: 80 (2.5 µg/ml) and 1: 200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100 and DAB.