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Item 1 of 4
Item 1 of 4
Nanos Homologue 1 (NANOS1) Antibody (C-term)
Catalog Number: orb1936151
| Catalog Number | orb1936151 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This Nanos Homologue 1 (NANOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the C-terminal region of human Nanos Homologue 1 (NANOS1). |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | Q8WY41 |
| MW | 30230 Da |
| Tested applications | FC, IF, IHC-P, WB |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Antibody Type | Primary Antibody |
| Clone Number | RB13839 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Nanos homolog 1, NOS-1, EC_Rep1a, NANOS1, NOS1 |
| Note | For research use only |
| NCBI | NP_955631.1 |
Western blot analysis of anti-NANOS1 Antibody (C-term) in T47D cell line lysates (35 ug/lane). NANOS1 (arrow) was detected using the purified Pab.
Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
