Nanog antibody

• Catalog Number: orb195357
• Category: Antibodies
• Description: Goat polyclonal antibody to Nanog
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Dilution range: ELISA: 1:128000, WB: 0.5-2 μg/ml
• Conjugation: Unconjugated
• MW: 34.6
• Target: Nanog (mouse aa270-284)
• Protein Sequence: DLEVNLEATRESHAH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Nanog; Nanog homeobox; 2410002E02Rik, ENK, ecat4;
• Note: For research use only
• Application notes: WB: Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 34.6kDa according to NP_082292.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zbinden et al, EMBO J. 2010 Aug 4;29(15):2659-74. PMID: 20581802). Recommended concentration: 0.5-2μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NIH3T3 lysate using Nanog antibody